Stable isotope labeling peptide synthesis

Isotopically labeled peptides differ from ordinary peptides by replacing C with C13 or N with N15 in one or more amino acids in their structure. As shown in the picture below:

Stable isotope labeling technology is an important means of biomedical engineering, which is widely used in drug development, protein research and metabolic kinetics. This technique uses isotopically labeled molecules to track the behavior of biomolecules, providing a very powerful tool for studying biological processes, drug metabolism, and disease mechanisms.

With the increasingly widespread and in-depth application of peptides in the field of biomedicine, the demand for labeled and modified peptide types is increasing, and the quality requirements are becoming higher and higher. Stable isotope labeling is a typical one.

Isotopically labeled peptides differ from ordinary peptides by replacing C with C13 or N with N15 in one or more amino acids in their structure. As shown in the picture below:

* The difficulty of isotope labeling polypeptides: how to use atomic labeling of isotopic amino acids at appropriate peptide chain sites.

At present, the isotopically labeled polypeptides synthesized by Motif are mainly C13 and N15 isotopically labeled polypeptides, and the purpose of effectively labeling the whole peptide chain is to directly introduce isotopically labeled amino acids into the peptide chain. Commonly used isotopically labeled amino acids include Tyr, Thr, Lys, Arg, Glu, etc. Precise use of atomic labeling of isotopic amino acids at appropriate peptide chain sites.

Motif has a senior research and development team, mature synthesis and purification technology, strict quality requirements, is an important guarantee that we can meet the different purity requirements of customers for isotopically labeled peptides, we provide quality services in the microgram, milligram to kilogram level.

At present, MOTIF has successfully synthesized sequences as follows: LA*IY**LR* (R* is isotopically labeled 13C, 15N) (see figure below)