Polypeptide protein coupling

Small peptides/haptens must be coupled to carrier proteins (KLH, BSA, Ova) before antibodies can be obtained. In general, polypeptides can be coupled to proteins under the following conditions: 1. There is a free amino or carboxyl group. 2. -SH on cysteine can also be coupled with carrier proteins. At present, our company provides high quality coupled carrier proteins (KLH, BSA, OVA).

Polypeptide antigens are too small to produce a significant immune response, so they need to be coupled to larger protein carriers such as KLH (Keyhole Limpet Hemocyanin), BSA (Bovine Serum Albumin), OVA (Ovalbumin). KLH has no inhibitory effect in ELISA or Western blotting detection, so it does not affect the detection results. The commonly used coupling principle is maleimide theory, which is the coupling of cysteine residues in polypeptides with carrier proteins. Therefore, the addition of cysteine residues in the N-terminal or C-terminal of antigen polypeptide is conducive to the coupling of polypeptide and carrier protein.

In general, polypeptides can be coupled to proteins under the following conditions:

(1) The sulfhydryl group (-SH) on Cys can be coupled (recommended)

(2) or have an unprotected (free) amino or carboxyl group

Peptide KLH coupling modification:

KLH is coupled to a polypeptide by n-terminal NH2

KLH is coupled to polypeptides by C-terminal -COOH

KLH is coupled to a thiol peptide by Cys

Polypeptide BSA coupling modification:

BSA is coupled to a polypeptide by n-terminal NH2

BSA is coupled to polypeptides by C-terminal -COOH

BSA is coupled to Cys by sulfhydryl polypeptides

Polypeptide OVA coupling modification:

The OVA is coupled to the polypeptide by the n-terminal NH2

OVA is coupled to polypeptides by C-terminal -COOH

OVA is coupled to Cys by sulfhydryl polypeptides

(1) Advanced synthesis technology and automation equipment can quickly and efficiently produce a large number of couplings to meet the needs of drug development and production.

(2) Customized design and production of specific types of couplings to meet the needs of different research and treatment.

(3) The quality control of the coupling is very strict, which can provide high-quality products and ensure the accuracy and reliability of production and research.

(4) To provide customers with a full range of services, including technical advice, product recommendation, after-sales service, etc., to help customers better use polypeptide protein coupling technology.

(5) For different coupling objects, we have different systematic coupling methods that can accurately couple proteins to peptides, and then remove the target peptides.

At present, MOTIF has been successfully synthesized sequence is: EG L * * * * * * * * * * country SV * * * * * * * * * * LS SDMD * * * * * * * * * * HC, coupling KLH (below)